제품설명

PicoSens™ Total Cholesterol Assay Kit는 흡광도 (Colorimetric) 및 형광 (Fluorescent) 측정의 정량 분석을 통해 Free cholesterol, Cholesteryl Esters 또는 모두를 검출하는 데 사용될 수 있습니다. 혈액 내 Cholesterol의 대부분은 Cholesteryl Esters에 의해 Free Cholesterol로 가수 분해되는 Cholesterol esterase 형태로 존재합니다. Cholesterol은 산화 효소에 의해 산화되어 부산물로서 H₂O₂를 생성하며, 이는 cholesterol probe와 반응하여 흡광 (OD = 570 nm) 또는 형광 (Ex/Em = 535/590 nm) 을 생성합니다.  

 

제품구성

결과분석

C (㎍/㎕) = B/V x D

C : Sample의 Cholesterol 농도 (㎍/㎕)
B : 측정 Well의 Cholesterol 양 (㎍)
V : Well에 분주한 Sample의 Volume (㎕)
D : Sample 희석 배율 

 

 

인용논문

1)Koh, Y.I., Oh, K.S., Kim, J.A., Noh, B., Choi, H.J., Joo, S.Y., Rim, J.H., Kim, H.-Y., Kim, D.Y., Yu, S., et al. (2022). OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin. Autophagy 18, 2593-2614. 10.1080/15548627.2022.2040891. 

2) Lee, D., Lee, K.-H., Kim, D.W., Yoon, S., and Cho, J.-Y. (2022). CXCL5 inhibits excessive oxidative stress by regulating white adipocyte differentiation. Redox Biology 54, 102359. https://doi.org/10.1016/j.redox.2022.102359. 

3) Lee, J., Jeong, I., and Kim, O.-K. (2022). Liver-Derived Exosomes Induce Inflammation and Lipogenesis in Mice Fed High-Energy Diets. Nutrients 14, 5124. 90. 

4) Park, S.-J., Kim, J.-L., Park, M.-R., Lee, J.W., Kim, O.-K., and Lee, J. (2022). Indian gooseberry and barley sprout mixture prevents obesity by regulating adipogenesis, lipogenesis, and lipolysis in C57BL/6J mice with high-fat diet-induced obesity. Journal of Functional Foods 90. 

5) Kwon, M., Lim, S.-J., Joung, E.-J., Lee, B., Oh, C.-W., and Kim, H.-R. (2018). Meroterpenoid-rich fraction of an ethanolic extract from Sargassum serratifolium alleviates obesity and non-alcoholic fatty liver disease in high fat-fed C57BL/6J mice. Journal of Functional Foods 47, 288-298. https://doi.org/10.1016/j.jff.2018.05.063.

6) Jung, D.Y., Suh, N., and Jung, M.H. (2022). Tanshinone 1 prevents high fat diet-induced obesity through activation of brown adipocytes and induction of browning in white adipocytes. Life Sci 298, 120488. 10.1016/j.lfs.2022.120488.