제품설명
ATP는 생물이 살아가는데 필요한 주요 에너지원입니다. 사실상 모든 대사와 생합성과정은 ATP의 인산염 결합에 저장된 화학 에너지를 이용합니다. BIOMAX PicoSens™ ATP Assay Kit는 존재하는 ATP의 양에 비례하여 흡광 (OD = 570 nm) 또는 형광 (Ex/Em = 535/590 nm) Product를 생성하며, Glycerol (Glycerol-6-Phosphate)을 인산화하여 ATP 농도를 결정하는 강력하고 간단한 방법으로 설계되었습니다.
제품구성
Components |
Size |
Storage |
---|
ATP Assay Buffer |
25 ㎖ |
-20℃ |
ATP Enzyme mix (Lyophilized) |
1 vial |
ATP Converter
|
200 ㎕
|
ATP probe |
200 ㎕ |
ATP Standard (10 mM) |
100 ㎕ |
* 개봉하지 않은 제품은 빛을 차단한 상태에서 -20℃ 보관 시 약 1년 간 안정적입니다.
결과분석
C (nmol/㎕ or μmol/㎖ or mM) = B/V x D
C : Sample의 ATP 농도 (nmol/㎕)
B : 측정 Well의 ATP 양 (nmol)
V : Well에 분주한 시료의 부피 (㎕)
D : Sample 희석 배율
인용논문
1) Kim, S., Oh, M., Kang, M., and Ko, J. (2022). Small leucine zipper protein functions as a modulator for metabolic reprogramming of colorectal cancer cells by inducing nutrient stress-mediated autophagy. Cellular and Molecular Life Sciences 79, 505. 10.1007/s00018-022-04535-4.
2) Song, N., Park, M., Kim, N., Lee, Y., Jung, E., and Lee, D. (2022). Tumor-targeting oxidative stress nanoamplifiers as anticancer nanomedicine with immunostimulating activity. Biomaterials Science 10, 6160-6171. 10.1039/D2BM00601D.
3) Sukhan, Z.P., Hossen, S., Cho, Y., Lee, W.K., and Kho, K.H. (2022). Hdh-Tektin-4 Regulates Motility of Fresh and Cryopreserved Sperm in Pacific Abalone, Haliotis discus hannai. Front Cell Dev Biol 10, 870743. 10.3389/fcell.2022.870743.
4) Lim, S.G., Suk, K., and Lee, W.H. (2020). LETMD1 Regulates Phagocytosis and Inflammatory Responses to Lipopolysaccharide via Reactive Oxygen Species Generation and NF-kappaB Activation in Macrophages. J Immunol 204, 1299-1309. 10.4049/jimmunol.1900551.
5) Chang, Y.B., Hong, K.B., Kim, M.G., Suh, H.J., and Jo, K. (2021). Effect of the protein hydrolysate of rice syrup meal on the endurance exercise performance of BALB/c mice. Food Funct 12, 1338-1348. 10.1039/d0fo02667k.
6) Sharma, S., Lee, K.-J., Bajgai, J., Trinh, T.T., Antonio, J.M., Rahman, M.H., Vira, K., Sofian, A.-N., Cho, S.H., Kim, C.-S., and Kim, Y. (2022). Anti-Oxidative and Anti-Diabetic Effects of Electrolyzed Weakly Alkaline Reduced Water on Renal Proximal Tubular Epithelial Cells. Processes 10, 2025.
7) Lee, Y., Saifuddin, M., Park, S.Y., and Kim, C.G. (2023). Synergistic effect of total residual oxidants and microplastics on seawater disinfection: An ecotoxicity study. Environmental Engineering Research 28, 210612-210610. 10.4491/eer.2021.612.10, 2025.